A New Generation of Matrix for Protein Purification Offers 3 High (3H)
A New Standard and Criteria for Chromatography Media
μSphere™微球是與Source相同的層析填料基材，是集高流速、高載量、高分辨率(3H)于一體的新一代蛋白純化填料的基礎(chǔ)材料。過(guò)去二十多年來(lái)，聚合物微球得到了極大發(fā)展并成功應(yīng)用于生物物質(zhì)分離和純化。PAE開發(fā)的聚合物微球，簡(jiǎn)寫μSphere™是用單分散高交聯(lián)度聚丙烯酸酯微球為基材并在其表面加特殊涂層并接枝功能基團(tuán)或配體制備的層析介質(zhì)。傳統(tǒng)的多糖基基材雖生物相容性好，但其骨架剛性低，在較高的壓力下容易出現(xiàn)變形或者孔道的改變，影響純化效率。μSphere™有效解決了瓊脂糖骨架剛性低的問(wèn)題，具有諸多更適于線性放大，加快工藝開發(fā)速度。不但適于實(shí)驗(yàn)室研究用樣品的制備，而且是工業(yè)規(guī)模純化蛋白、多肽、核酸、抗生素和低分子量藥物不可回避的選擇，且 PAE生產(chǎn)的用于生物大分子分離的微球?yàn)槎嗫椎模卸喾N粒徑和孔徑，可達(dá)1000A,根須需求接上-OH,-COOH或-NH2 等基團(tuán)，一邊方便偶聯(lián)其它配體或配基。粒徑/孔徑默認(rèn)50mm，粒徑800 ?。可按客戶要求提供粒徑15mm，30mm，100mm和孔徑300、500和800的產(chǎn)品。
高流速
μSphere具有很高的機(jī)械穩(wěn)定性且粒徑單一，因此層析特性較好，具有極很好的抗壓縮性能，不易出現(xiàn)堵塞。另外，其可耐操作過(guò)程中高至10MPa的壓力，支持高達(dá)2000cm/hour的流速。
高載量
μSphere是一種可精確控制孔徑和粒徑的單分布多孔微球體，其比表面積高達(dá)>500m2/g,用其制備的層析填料具有很高的功能基團(tuán)密度，其動(dòng)態(tài)載量。圖1是NTA-μSphere和NTA-Sepharose FF對(duì)比15個(gè)His-標(biāo)簽蛋白的載量分布。圖中藍(lán)色柱代表NTA-μSphere對(duì)某樣品的載量占NTA-Sepharose FF載量的倍數(shù)，紅線是NTA-Sepharose FF的相對(duì)載量等高線，可見NTA-μSphere顯著超過(guò)NTA-Sepharose FF。
高分辨率
μSphere的粒徑相對(duì)較小(50μm±5%)且分布相當(dāng)均一，機(jī)械強(qiáng)度高，不易變形，因此分辨率明顯高于糖基微球。圖2是NTA-μSphere和NTA- Sepharose FF上樣量等條件均一條件下的洗脫峰，顯然，NTA-μSphere的峰形更"sharp"。
其他特點(diǎn)
除上述3H外，mSphere在pH1到14都是穩(wěn)定的，因此可以用0.5 MNaOH甚至更高的堿濃度進(jìn)行在位清洗(CIP)。堿液在位清洗是科研產(chǎn)品除熱源以及雜蛋白的方法。
Appendix
下表是mSphere與Agarose微球與蛋白純化有關(guān)的理化性質(zhì)的比較。從中可以看出，mSphere具有多方面的優(yōu)勢(shì)。
主要基材類型總結(jié)與對(duì)比
類型 | 基材 | 粒徑(μm） | pH穩(wěn)定性 | 壓力 | 流速(cm/h) |
Source 15 | PS/DVB | 15 | 1~14 | 10MPa | 900 |
Source 30 | PS/DVB | 30 | 1~14 | 10 | 2000 |
Resource | PS/DVB | 15 | 1~14 | 10 | 1800 |
MonoBeads | Monodisperse porous PS/DVB | 10 | 1~14 | 10 | 1200 |
MiniBeads | Monodisperse PS/DVB | 3, non porous | 1~14 | 10 |
|
Fast Flow | 6% cross-linked agarose | 45-165, avr. 90 | 2~13 | 0.3 | 300 |
Capto | Highly cross-linked agarose with dextran surface extender | 90 (d50V) | 2~13 | 0.3 | 1200 |
HP | 6% cross-linked agarose | 24-44, avr. 34 | 1~14 | 0.3 | 150 |
mSphere | Polyacrylate or PS/DVB | 3, 10, 15, 30, 50, 100 | 1~14 | 10 | 1200~2000 |
P022 SP-μSphere
SP-聚合物微球，強(qiáng)陽(yáng)離子交換樹脂
載量可與GE比肩，強(qiáng)度、流速、分辨率高于GE等公司以瓊脂糖為基材的產(chǎn)品SPμSphere is a strong cation exchanger based on the well established μSphere ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
- Well-proven strong cation exchanger developed for industrial downstream processes.
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
SP μSphere is part of the μSphere ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of cross-linked, 6% polymer particles, with sulphopropyl (SP) strong cation exchange groups. SP μSphere has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with SP μSphere is straight forward and the resin is available in a range of pre-packed process development tools.
As member of the BioProcess resin range, SP μSphere meets industrial demands with security of supply and comprehensive technical and regulatory support.
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