PC019 HiTalon SμSphere預裝柱
特點：
1. 尺寸和接口與HiTrap一致，無死體積。
2. 直連GE、waters、Agilent等的HPLC，如Akta。
3. μSphere為基材的聚合物填料，有高流速、高載量、高分辨，顯著優于瓊脂糖膠。
? prepared with columns identical to HiTrap, no dead volume.
? can be directly connect to HPLC from GE, waters,Agilent, such as Akta quickly.
? The basal matris developed by pae provides High flow rate, High dynamic binding capacity and High performance.
All these are very important for chromatography. For details of the matrix, please see P019.
HiTrap SμSphere is prepacked with S μSphere, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
l Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
l The industry standard for ion exchange chromatography.
l High flow rates and good scale-up potential.
l Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
l Predictable scale-up
Q, SP, DEAE adn CM μSphere are based on a robust, highly cross-linked monodispersed beads with high flow rates, high loading capacities and high resolution .their performance is much more better that Sepharose based on highly cross-linked beaded agarose.
About*S μSphere
S μSphere is a strong cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
l Well-proven strong cation exchanger developed for industrial downstream processes.
l Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
l The industry standard for ion exchange chromatography during recent decades
l High chemical stability allows for well proven CIP and sanitization protocols
l The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
S μSphere is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of cross-linked, 6% agarose particles, with sulphopropyl (SP) strong cation exchange groups. S μSphere has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with S μSphere is straight forward and the resin is available in a range of pre-packed process development tools.
As member of the BioProcess resin range, S μSphere meets industrial demands with security of supply and comprehensive technical and regulatory support.
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