Phospho-JAK2 (Tyr1007/1008) Rabbit Monoclonal Antibody
產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝 |
AF1486 | Phospho-JAK2 (Tyr1007/1008) Rabbit Monoclonal Antibody | 50µl |
產(chǎn)品簡介：
來源 | 用途 | 交叉反應(yīng)性 | 分子量 |
Rabbit | WB, IP, IF, IHC, ICC | H, M, R | 130 kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, ; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, ; , .
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
?配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當(dāng)用途時(shí)的一抗稀釋。
WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
1:500-1:1,000 | 1:10-1:50 | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 | - | - | - |
?建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
?抗體詳細(xì)信息如下:
About this Antibody | |
Name | Phospho-JAK2 (Tyr1007/1008) Rabbit Monoclonal Antibody |
Category | Rabbit Monoclonal Antibody (RabMAb); Primary antibody |
Isotype | IgG |
Purification | Affinity purification |
About the Immunogen | |
Immunogen | Recombinant protein |
Gene ID | 3717(Human); 16452(Mouse); 24514(Rat) |
SwissProt | O60674(Human); Q62120(Mouse); Q62689(Rat) |
Synonyms | JTK10; THCYT3 |
Category | Immunology/Inflamation |
Background | JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immuno-logical stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK-Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering JAK-Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 are sites of trans- or autophosphorylation in vivo and in in vitro kinase reactions. |
包裝清單：
產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝 |
AF1486 | Phospho-JAK2 (Tyr1007/1008) Rabbit Monoclonal Antibody | 50µl |
AZ050 | Western一抗稀釋液 | 50ml |
— | 說明書 | 1份 |
保存條件：
Phospho-JAK2 (Tyr1007/1008) Rabbit Monoclonal Antibody -20oC保存，Western一抗稀釋液-20oC或4oC保存，一年有效。Western一抗稀釋液優(yōu)先推薦4oC保存，長期不使用可以考慮-20oC保存，但凍融可能會導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng)：
?如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4oC保存。
?回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
?提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
?本產(chǎn)品于專業(yè)人員的科學(xué)研究用，不得用于任何其他用途，不得存放于普通住宅內(nèi)。
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