Illumina/TruSeq Small RNA Set A MiniSeq Kit (1 library prep and 2 MiniSeq reagents)/20005613/1 Ea

美國Illumina公司

美國Illumina公司

Illumina公司資料

組建人員

Illumina是由David Walt博士、CW 集團的Larry Bock、獸醫學博士John Stuelpnagel、 Anthony Czarnik博士及Mark Chee博士于1998年4月共同組建。

技術開發

在與CW 集團風險投資公司合作期間，Larry和John在Tufts大學研究出了BeadArray技術，經過商議他們取得了該技術的專屬。

上市時間

他們把Illumina的總部設于加利福尼亞的圣地亞哥，并于2000年7月公開上市。

Product Highlights:
TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. CompatIBLe applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.

TruSeq Small RNA Sample Datasets
The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:
View Run (primary analysis and metrics)
View Project (secondary analysis with BaseSpace Apps)
Specifications:
Assay Time ~10.5 hours
Hands-On Time ~30 minutes
Input Quantity 25 to 100 ng total RNA from species with polyA tails
Content Specifications Captures the coding transcriptome with strand information
Mechanism of Action Oligo-dT beads capture polyA tails
Multiplexing Up to 24-plex pooling with additional NeoPrep runs
Sample Throughput Up to 16 samples per NeoPrep library card
Species Category Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode
System Compatibility MiSeq,NextSeq 550,MiniSeq,NextSeq 500
Specialized Sample Types FFPE,Low Input
Technology Sequencing
Automation CapABIlity Liquid Handling Robots
Variant Class Novel Transcripts,Transcript Variants
Method miRNA and Small RNA Sequencing