足底熱刺痛儀主要通過(guò)Hargreaves法檢測(cè)動(dòng)物縮足潛伏期PWL。

疼痛甩尾和冷熱板實(shí)驗(yàn)雖是急性疼痛熱閾值的經(jīng)典測(cè)量方法這兩種實(shí)驗(yàn)至今仍然被藥理學(xué)研究采用。但這兩種方法都有一些局限性沒(méi)有在痛覺(jué)過(guò)敏的行為反應(yīng)研究中得到運(yùn)用。

足底測(cè)試代表了一種的實(shí)驗(yàn)方法它集合了疼痛過(guò)敏測(cè)試的優(yōu)點(diǎn)

· 實(shí)驗(yàn)時(shí)受試動(dòng)物無(wú)拘束可自由活動(dòng)；

· 實(shí)驗(yàn)數(shù)據(jù)記錄是儀器自動(dòng)感應(yīng)完成的無(wú)需人為判斷和記錄；

· 通過(guò)聚焦紅外光源于動(dòng)物足底按下開(kāi)關(guān)等待動(dòng)物縮回受測(cè)足爪儀器可自動(dòng)記錄紅外光強(qiáng)度和持續(xù)時(shí)間；

· 紅外光源設(shè)置了一個(gè)特殊的過(guò)濾器能夠過(guò)濾掉可見(jiàn)光譜防止可見(jiàn)光干擾動(dòng)物影響實(shí)驗(yàn)結(jié)果；

· 帶自檢裝置反饋電路能夠進(jìn)行自檢能有效避免錯(cuò)誤的實(shí)驗(yàn)環(huán)境；

· 實(shí)驗(yàn)數(shù)據(jù)會(huì)顯示在液晶屏上數(shù)據(jù)可導(dǎo)入U盤(pán)或通過(guò)USB數(shù)據(jù)線導(dǎo)入至電腦。

· 文獻(xiàn)引用量超過(guò)2000的足底熱刺激設(shè)備；

· 數(shù)據(jù)在前板上顯示可以通過(guò)USB傳送到電腦上USB儲(chǔ)存設(shè)備和軟件都包含在標(biāo)準(zhǔn)的配件包里

型號(hào)37370

產(chǎn)品特點(diǎn)
· 可自動(dòng)或手動(dòng)記錄爪縮回時(shí)間不需要視覺(jué)評(píng)分無(wú)差錯(cuò)測(cè)量精確
· 觸摸屏控制所有功能和結(jié)果查看
· 配備USB接口可單獨(dú)工作也可連接電腦使用
· 帶數(shù)據(jù)統(tǒng)計(jì)軟件可將CSV文件從直接導(dǎo)出到USB
· 爪縮回潛伏期的分辨率為0.1s
· 紅外光強(qiáng)度01-99 級(jí)間可調(diào)
· 可選配紅外熱輻射校準(zhǔn)儀用于校準(zhǔn)紅外光源
· 6只大鼠或12只小鼠同時(shí)進(jìn)行實(shí)驗(yàn)

主機(jī)及測(cè)試光源

主要參數(shù)

· 操作方式按鍵
· 數(shù)據(jù)讀取液晶屏顯示
· 紅外光強(qiáng)度01-99 級(jí)間可調(diào)
· 時(shí)間分辨率0.1s
· 紅外燈泡Halogen "Bellaphot", Mod. 64607 OSRAM, 8V-50W
· 數(shù)據(jù)轉(zhuǎn)移閃存
· 電源85-264 VAC, 50-60Hz
· 工作溫度15°- 30°C
· 噪音< 70dB
· 校準(zhǔn)紅外輻射計(jì)
· 規(guī)格85x40x35 cm
· 鼠籠尺寸20x20x14cm數(shù)量3個(gè)
· 凈重13.0kg

可選配Durham大鼠束縛器配合足底熱點(diǎn)儀用于大鼠下頜部三叉神經(jīng)痛測(cè)試。

刺激強(qiáng)度值（紅外熱輻射值）對(duì)照表

（單位mW/cm2）

主要配置清單

可選配紅外熱輻射測(cè)量?jī)x

足底熱刺痛儀的部分引用文獻(xiàn)

《Science》
1.La Montanara, Paolo, et al. "Cyclin-dependent–like kinase 5 is required for pain signaling in human sensory neurons and mouse models." Science translational medicine 12.551 (2020)eaax4846.doi:10.1126/scitranslmed.aax4846 
IF 19.32
2.Feng, Jiao, et al. "A new painkiller nanomedicine to bypass the blood-brain barrier and the use of morphine." Science advances 5.2 (2019)eaau5148.doi10.1126/sciadv.aau5148 
IF 14.96
3.Hsiao, Hung-Tsung, et al. "The analgesic effect of propofol associated with the inhibition of hypoxia inducible factor and inflammasome in complex regional pain syndrome." Journal of medical science 26 (2019)1-11. doi:10.1186/s12929-019-0576-z 
IF 12.77
4.Zhou, Luming, et al. "Reversible CD8 T cell–neuron cross-talk causes aging-dependent neuronal regenerative decline." Science 376.6594 (2022)eabd5926. doi10.1126/science.abd5926
IF 63.71

《Nature》

5.Oswald, Manfred J., et al. "Cholinergic basal forebrain nucleus of Meynert regulates chronic pain-like behavior via modulation of the prelimbic cortex." Nature Communications 13.1 (2022)5014.doi: 
IF 17.69
6.Landra-Willm, Arnaud, et al. "A photoswitchable inhibitor of TREK channels controls pain in wild-type intact freely moving animals." Nature Communications 14.1 (2023)1160.doi: 
IF 17.69
7.Nees, Timo A., et al. "Role of TMEM100 in mechanically insensitive nociceptor un-silencing." Nature Communications 14.1 (2023)1899.
doi10.1038/s41467-023-36806-4
IF 17.69
8.Zhang, Qiaosheng, et al. "A prototype closed-loop brain–machine interface for the study and treatment of pain." Nature Biomedical Engineering (2021)1-13. doi10.1038/s41551-021-00736-7
IF 29.23
9.Zhang, Su-Bo, et al. "CircAnks1a in the spinal cord regulates hypersensitivity in a rodent model of neuropathic pain." Nature communications 10.1 (2019)4119.doi:1
10.IF17.69  
11.Jiang, Wenhao, et al. "PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis." Bone research 10.1 (2022)27. doi:10.1038/s41413-022-00201-4
13.36
12.Bao, Yi-Ni, et al. "The dopamine D1–D2DR complex in the rat spinal cord promotes neuropathic pain by increasing neuronal excitability after chronic constriction injury." Experimental & Molecular Medicine 53.2 (2021)235-249.doi:10.1038/s12276-021-00563-5
IF 12.15
13.Takeda, Ikuko, et al. "Controlled activation of cortical astrocytes modulates neuropathic pain-like behaviour." Nature communications 13.1 (2022)4100.doi10.1038/s41467-022-31773-8
IF 17.69
14.Liang, Hai-Ying et al. “nNOS-expressing neurons in the vmPFC transform pPVT-derived chronic pain signals into anxiety behaviors." Nature communications vol. 11,1 2501. 19 May. 2020, doi:10.1038/s41467-020-16198-5 doi:10.1038/s41467-020-16198-5
IF 17.69
15.Zhou, Hang, et al. "A sleep-active basalocortical pathway crucial for generation and maintenance of chronic pain." Nature Neuroscience (2023)1-12. doi10.1038/s41593-022-01250-y
IF 28.77
16.Wang, Yan et al. “TRPV1 SUMOylation regulates nociceptive signaling in models of inflammatory pain." Nature communications vol. 9,1 1529. 18 Apr. 2018, doi10.1038/s41467-018-03974-7 
IF 17.69
17.Iwasaki, Mai, et al. "An analgesic pathway from parvocellular oxytocin neurons to the periaqueductal gray in rats." Nature Communications 14.1 (2023)1066. doi:10.1038/s41467-023-36641-7
IF 17.69

《Cell》
18.Zhang, Fang-Xiong et al. “BK Potassium Channels Suppress Cavα2δ Subunit Function to Reduce Inflammatory and Neuropathic Pain." Cell reports vol. 22,8 (2018)1956-1964. doi:10.1016/j.celrep.2018.01.073 
IF 10.00
19.Gui, Xianwei et al. “Botulinum toxin type A promotes microglial M2 polarization and suppresses chronic constriction injury-induced neuropathic pain through the P2X7 receptor." Cell & science vol. 10 45. 23 Mar. 2020, doi:10.1186/s13578-020-00405-3

方法學(xué)文獻(xiàn)

K.M. Hargreaves, R. Dubner, F. Brown, C. Flores and J. Joris"A New and Sensitive Method for Measuring Thermal Nociception in Cutaneous Hy-peralgesia" Pain 3277-88, 1988

D.C. Yeomans & H.K. Proudfit"Characterization of the Foot Withdrawal Response to Noxious Radiant Heat in the Rat" Pain 5985-97, 1994